PhytophthoraV1, Main, Exploration, bibRecord, 000400

Rapid and equipment-free detection of Phytophthora capsici using lateral flow strip-based recombinase polymerase amplification assay.

Identifieur interne : 000400 ( Main/Exploration ); précédent : 000399; suivant : 000401

Rapid and equipment-free detection of Phytophthora capsici using lateral flow strip-based recombinase polymerase amplification assay.

Auteurs : J. Yu [République populaire de Chine] ; D. Shen [République populaire de Chine] ; T. Dai [République populaire de Chine] ; X. Lu [République populaire de Chine] ; H. Xu [République populaire de Chine] ; D. Dou [République populaire de Chine]

Source :

Letters in applied microbiology [ 1472-765X ] ; 2019.

RBID : pubmed:31021437

Descripteurs français

KwdFr :
- Alkyl et aryl transferases (génétique), Amorces ADN (génétique), Limite de détection (MeSH), Maladies des plantes (parasitologie), Phytophthora (génétique), Phytophthora (isolement et purification), Recombinases (analyse), Sensibilité et spécificité (MeSH), Techniques d'amplification d'acides nucléiques (méthodes).
MESH :
- analyse : Recombinases.
- génétique : Alkyl et aryl transferases, Amorces ADN, Phytophthora.
- isolement et purification : Phytophthora.
- méthodes : Techniques d'amplification d'acides nucléiques.
- parasitologie : Maladies des plantes.
- Limite de détection, Sensibilité et spécificité.

English descriptors

KwdEn :
- Alkyl and Aryl Transferases (genetics), DNA Primers (genetics), Limit of Detection (MeSH), Nucleic Acid Amplification Techniques (methods), Phytophthora (genetics), Phytophthora (isolation & purification), Plant Diseases (parasitology), Recombinases (analysis), Sensitivity and Specificity (MeSH).
MESH :
- chemical , analysis : Recombinases.
- chemical , genetics : Alkyl and Aryl Transferases, DNA Primers.
- genetics : Phytophthora.
- isolation & purification : Phytophthora.
- methods : Nucleic Acid Amplification Techniques.
- parasitology : Plant Diseases.
- Limit of Detection, Sensitivity and Specificity.

Abstract

Phytophthora capsici is an important oomycete pathogen that causes devastating diseases in various crops. Methods for the rapid detection of P. capsici are important for disease control and eradication programmes. Here, we developed an assay based on lateral flow strip-based recombinase polymerase amplification (LF-RPA) for the rapid and equipment-free detection of P. capsici. The specific primers and a probe were designed using the sequence of Ypt1, and the optimal assay conditions were 40°C for 20 min. The specificity of the assay was verified using closely related oomycetes and fungal species, and its detection limit was 10 pg of genomic DNA. In combination with a simple DNA extraction method, the LF-RPA assay enabled detection of P. capsici in diseased pepper samples without specialized equipment within 30 min. Consequently, the LF-RPA assay developed in this study enables rapid and equipment-free detection of P. capsici and has potential for further development as a diagnostic kit for application in the field or in resource-limited laboratories. SIGNIFICANCE AND IMPACT OF THE STUDY: We developed a novel assay based on lateral flow strip-based recombinase polymerase amplification (LF-RPA) for the rapid and equipment-free detection of Phytophthora capsici. In combination with a simple DNA extraction method, the LF-RPA assay detected P. capsici in field samples without specialized equipment within 30 min. The assay has potential for further development as a diagnostic kit for application in the field or in resource-limited laboratories.

DOI: 10.1111/lam.13166
PubMed: 31021437

Affiliations:

République populaire de Chine

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000490
to stream Main, to step Curation: 000490

Le document en format XML

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<term>Phytophthora (isolement et purification)</term>
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<front><div type="abstract" xml:lang="en">Phytophthora capsici is an important oomycete pathogen that causes devastating diseases in various crops. Methods for the rapid detection of P. capsici are important for disease control and eradication programmes. Here, we developed an assay based on lateral flow strip-based recombinase polymerase amplification (LF-RPA) for the rapid and equipment-free detection of P. capsici. The specific primers and a probe were designed using the sequence of Ypt1, and the optimal assay conditions were 40°C for 20 min. The specificity of the assay was verified using closely related oomycetes and fungal species, and its detection limit was 10 pg of genomic DNA. In combination with a simple DNA extraction method, the LF-RPA assay enabled detection of P. capsici in diseased pepper samples without specialized equipment within 30 min. Consequently, the LF-RPA assay developed in this study enables rapid and equipment-free detection of P. capsici and has potential for further development as a diagnostic kit for application in the field or in resource-limited laboratories. SIGNIFICANCE AND IMPACT OF THE STUDY: We developed a novel assay based on lateral flow strip-based recombinase polymerase amplification (LF-RPA) for the rapid and equipment-free detection of Phytophthora capsici. In combination with a simple DNA extraction method, the LF-RPA assay detected P. capsici in field samples without specialized equipment within 30 min. The assay has potential for further development as a diagnostic kit for application in the field or in resource-limited laboratories.</div>
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{{Explor lien
   |wiki=    Bois
   |area=    PhytophthoraV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:31021437
   |texte=   Rapid and equipment-free detection of Phytophthora capsici using lateral flow strip-based recombinase polymerase amplification assay.
}}

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       | NlmPubMed2Wicri -a PhytophthoraV1

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	Serveur d'exploration Phytophthora
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Serveur d'exploration Phytophthora

Rapid and equipment-free detection of Phytophthora capsici using lateral flow strip-based recombinase polymerase amplification assay.

Rapid and equipment-free detection of Phytophthora capsici using lateral flow strip-based recombinase polymerase amplification assay.

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